Fabio Biella

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Questions related from Fabio Biella

How to solve coating issues between primary and iPSC-derived neurons?

Dear all, I have issue with cells detaching during PFA fixation and washes. Following a paper's instruction we are differentiation neurons from iPSC and then plate them on PDL+Laminin coating,...

06 February 2025 5,125 1 View

How to prepare proper Borate buffer for neuron coating?

Dear all, replicating a protocol from a paper I have to prepare a borate buffer to dilute poly-D-lysine for a coating. The paper shows this recipe: Boric acid 100 mM Sodium tetraborate 25...

06 February 2025 1,181 4 View

BED enhancer to tissue expression. How to?

Hi everyone! I have a list of genomic features in BED file format. They represent short features which are enhancer or silencer. I have to pick some for further investigation and I decided to...

25 September 2023 7,187 0 View

PAXgene blood miRNA kit with debries?

Hi everyone! I'm using PAXgene blood tube and PAXgene blood miRNA kit for RNA extraction from blood samples. Tubes have been stored at -80°C but when i thaw them some contain dark brown sand-like...

25 September 2023 2,606 0 View