We are currently culturing several bacterial strains which grow as clumps in liquid medium (without agitation because they are micro-aerophillic) for CFU counting at a given OD (0.8 for instance), but we are struggling to obtain an homogenous suspension. They apparently produce a lot of extracellular matrix. We tried to first pellet the bacterial cultures and then disrupt the bacterial pellets in as little PBS as possible by harsh pipetting and sonication (ultrasonic bath) but it does not work.
Do you have any suggestions/tips?
David Lepetit
Have you considered doing gentle (-partial-incomplete) digestions with some kind of "Enzymatic dispersion of biofilms".
Best wishes
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