Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

Which kind of validity should e established in case we want to conduct a survey using newly developed survey questionnaire?

I am conducting a survey using self developed questionnaire. I have established its face validity and content validity. Some expert reviewer has suggested I must establish the validity by...

11 February 2021 2,077 3 View

How to calculate width and length of the tapered microstrip line feed of a SIW leaky wave antenna?

With reference to substrate integrated waveguide -leaky wave antenna

26 December 2020 3,982 2 View

Sample does no retain in the well while doing NATIVE Gel electrophoresis?

While doing NATIVE-Gel electrophoresis with electrophoresis buffer containing 25mM Tris, 192 mM glycine, and rest water if the sample being loaded is dissolved in methanol it won't remain in the...

15 December 2020 4,999 3 View

How to identify and quantify glauconite and illite/other mica present in same sample from XRD analysis?

In glauconite bearing sand stone sample other mica group minerals(illite, muscovite etc.) are also present. I want to quantify the amount of glauconite and othermica group minerals. How, I can...

10 December 2020 5,793 19 View

Collaborating VS contributing Authorship?

Can you help me to understand the significance of the difference between collaborating and contributing authorship (eg, GBD)? Do they put similar weightage to University applicant assessors?

29 October 2020 4,443 3 View

How to prevent leaks in Swagelok fittings?

I am carrying out a liquefaction reaction inside a 2.5mL volume reactor made of Swagelok fittings (one SS 3/4" STRAIGHT UNION with two SS 3/4" PLUGS FOR FITTING). After adding the substrate...

11 August 2020 4,211 2 View

How to prevent lignin condensation during biomass fractionation?

10 August 2020 4,936 1 View

How the Natural Language processing successful without knowledge of sense behind the letters and numbers ?

We don't know the source of letters and numbers on which these symbols are constructed . Although human used the guts feelings at the time of using because our ancestors developed these symbols...

01 August 2020 2,148 4 View

How to add attenuation model and spectral ordinate in R-CRISIS?

Hi Everyone I am using R-CRISIS for seismic hazard map creation. I know how to use the given attenuation model in R-CRISIS but I want to use an attenuation model from a published article. How to...

23 July 2020 5,265 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Negative result on natural product sample cholinergic assay?

i have been doing cholinergic assay on natural product and for some of the samples, i get negative value for the inhibition activity. can anyone help me explain the meaning of the negative value...

17 February 2021 4,118 1 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View