Could some advise me from where can I obtain P22 phage in India?
I am working with Amaranth accessions which are green to red in color and contain high phenolic compounds. Using the CTAB method with the slight modification I am trying to get high-quality DNA so...
18 January 2021 3,578 1 View
I am working with h-BN and need to find out the strain due to in-plane lattice constant. The famous Williamson-Hall method may not be applicable here as it analyzes XRD for calculation of strain...
19 October 2020 7,386 4 View
Hi all, I am using the following code to obtain the bar plot uisng the R package Ggpubr. I am required to add texture into the bar plots instead of color. I could not find any option for adding...
29 July 2020 829 3 View
I ran the response surface modeling using central composite design. And I got this output. Can I take this model? The model is having lack of fit p value more than 0.05. But other parameters are...
23 February 2020 6,776 4 View
For instance, both nitride NW-QDs and h-BN point defect based SPEs seem to exhibit photon entanglement. Which platform will provide stronger entanglement? I suspect, that purity of the single...
19 January 2020 6,442 2 View
I am a student and currently have no access to funds. I have extend my previous work ( book chapter) and interested in submitting to a journal. Can anyone please suggest to me some journals that...
25 December 2019 2,239 4 View
What is the reason that most of the researcher uses mice for evaluating immune response of vaccines. Why nobody uses rat for the same purpose?
17 November 2019 1,203 3 View
HOPG is a good substrate for growth of h-BN. One major application of h-BN is single photon emission which can be used for telecom wavelength. Can anyone give me any insight about the absorption...
11 September 2019 6,306 1 View
I am writing a paper on NLP related problem. I used deep learning, and this was never used before for this specific problem. Apparently the accuracy decreases if the number of data increases. I...
28 July 2019 3,870 34 View
Since humanized and SCID mice are very expensive to procure and maintain. I was wondering to develop a cost and effective approach to develop an in vivo model for cancer research. Thymectomy is...
10 July 2019 8,111 4 View
based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...
26 February 2021 6,799 3 View
Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...
22 February 2021 9,969 3 View
Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...
21 February 2021 7,260 4 View
When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...
21 February 2021 6,084 5 View
I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...
17 February 2021 7,029 3 View
I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...
16 February 2021 8,519 5 View
Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...
15 February 2021 9,563 6 View
When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...
09 February 2021 5,423 1 View
Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...
09 February 2021 4,994 1 View
My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....
08 February 2021 5,965 1 View