Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

Do you know any free clinical Covid19 database available?

Hello, everybody. I would like to apply machine learning techniques to clinical data of patients with Covid19. However, I can not find any clinical database of Covid patients, do you know any...

02 December 2020 706 8 View

Thermo LCQ Advantage unstable mass reading by electronics failure?

Hi there. Operation of a Thermo LCQ Advantage was perfect until turned it to stand-by. Next day, the system was turned on and everything seemed fine. However, TIC signal completely disappeared in...

06 July 2020 3,119 2 View

What sample bag should be use for soil sampling?

The samples will be analyze for microplastics. I have found research articles that used plastic bags or aluminum bags.

30 April 2020 9,435 13 View

Field studies of nearest neighbor distance: how do you handle cases with no neighbors in sight?

In studies in which one of the variables recorded is the minimum neighbor distance of a number of focal animals, how do people handle cases where a neighbor cannot be identified? Excluding them...

25 April 2020 1,116 5 View

Which contrast agent would you choose to assess animal vasculature by histology in the context below?

Dear all, I would like to assess by histology the degree of vascularisation that occurs on a mouse bone after the local administration of a certain compound. I've thought to administer a contrast...

01 September 2019 3,689 1 View

How can I recode multiple variables into one variable with specific conditions in SPSS?

Hello, I would appreciate your helping recoding multiple variables into a new variables with 4 levels but using certain conditions for each level. I have 2 variables, (VigPA, ModPA), each with a...

20 August 2019 8,455 3 View

How I can program a PIC?

Hello. My name is Enrique Mesa. I am looking for ways to program a PIC microcontroller at home. I am a student planning to do a project this semester for university. I plan to purchase a...

15 June 2019 7,298 5 View

Hello, any suggest for a research in iot security ?

I need to define a topic,It should not be so extensive to be able to cover in a good way.My idea is to make a security comparison between two IoT technologies,however i feel the topic stills too...

12 June 2019 3,221 3 View

Why are the journals of negative results not succeeding?

Hi, I have been considering to publish my null results since I think it can also contribute to science and also save researchers' money and time. I have found a couple of journals of negative...

01 February 2019 5,679 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

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28 February 2021 9,936 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View