Hello everyone, I am checking the quality of some RNA-seq data with FASTQC and I am getting results that are not clear to me. Is this kind of result normal?
What type of sample is this, it sure isn't patient-derived FFPE. I would expect even a cell line sample to show box and whiskers plot around Q30. The blue line I think indicates average and the red lines that we can see at the very top are I am guessing the median value that would appear in the box and whiskers plots. The reads, which sequencing device produced them?
Please provide more information. If it is fastqc report share the whole report.
From this graph, your data is surprisingly a very high quality.
The plot shows that the average quality per base alongside your 150 pb reads is very high which is good. This result is kind of normal when the sequencing has been outsourced. Most companies will give you prefiltered fastq files containing only reads with high quality. You can ask your sequencing provider if that was the case, although sometimes you can also find that info in the report they send together with your fastq files.
Marlene Jara Thank you very much for your valuable comments. I'll ask for the sequencing report.
I agree with Marlene, this is likely prefiltered data, which is why it looks so clean.
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