In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

Can I use saponin to permeabilize cell to measure mitochondrial respiration?

Saponin and digitonin both can permeabilize cells. However, based on many respiration assay protocols I read, saponin is used for tissue permeabilization and digitonin is used for cell...

11 January 2021 2,151 3 View

How can I incorporate data with different measurement frequencies into one structural equation model (SEM)?

09 December 2020 6,240 4 View

Are there any sound of respiration datasets related to COVID-19?

I try to analyze the respiration of humans that infected with COVID-19, Anyone has the dataset of that disease? we seek cooperation with scientific research.

20 May 2020 1,080 2 View

How can I design an IoT Platform for medical applications and Healthcare?

Hi. I want to design an IoT platform for healthcare and medical applications. I want to involve different people for different tasks. But I lack methodology and a clear roadmap. If anyone can help...

09 October 2019 5,247 2 View

Looking for a paper: what happens to E. coli in CO2 environment?

Hello, I've been looking around but can't seem to find a solution to a problem. I want to understand how does E. coli respond to CO2 in their environment (0-10% CO2). I know they can change their...

06 July 2019 2,622 4 View

How to measure atmospheric CO2 with GC-TCD ?

Hi all, actually im trying to develop a method to measure moss microbial biomass by substrate-induced respiration (the production of Co2). My problem is when I try to do a blank by injecting air...

06 July 2019 9,078 3 View

Are there methods to stabilize resorufin in a resazurin-based cell viability assay?

I am developing a cell viability assay where the decay in resorufin fluorescence (due to conversion to hyrdoresorufin) results in a narrow window in which to read out assay results. I am not...

05 June 2019 888 1 View

Alternative regression way to analyze relationship between parameters in a limited data?

Hi all, I have a data set, including soil respiration and carbon amount. After plotting a scatter plot respiration vs carbon amount, I can see there is kind of positive relationship between them....

05 June 2019 7,983 5 View

What would be a good Rhod-2 calibration buffer for mitochondrial matrix?

I am wondering whether regular mitochondrial respiration buffer, i.e., 120 mM KCl, 10 mM MOPS, 5 mM KH2PO4, pH7.25 would be OK to be used as calibration buffer for Rhod-2?

04 May 2019 3,455 1 View

Is it possible we get Non- trivial Berry phase with High oscillatory frequency ?

If the extracted oscillatory frequency (SdH) is more than 1000 T,so what are the chances we can get the Non-trivial Berry phase with such a high frequency ? I will really appreciate if someone...

22 April 2019 7,568 1 View