I am looking for method of analysis of trimethyl benzene and trioctyl phosphate on HPLC.Is it possible to determine these componets on HPLC,I have the technique to analyse on GC but i am searching for HPLC?
Dear Rehan,
yes. this is possible,
please tell us whether you are going to do assay of these compounds with simple matrix or high matrix, or going to do purity / related substances determination?
Regards
Selvan
like i have solution of ethyl anthra quinone ,trimthyl benzene,trioctyl phosphate and i am am analysing EAQ on HPLC but unable to analyze solvents on HPLC.
Dear Rehan,
Please try the below method
Column : any C18 Phase (ODS)
Mobile Phase : 90% Water and 10% Acetonitrile
Wavelength : 210 nm
for best chromatography the retention time of main peak should be more than 5.0 min with good peak shape. optimize the mobile phase if required
note: GC is the best way for solvents
Selvan
Yes GC is the best Method.Regarding your suggestion I have C18 column and i tried with the same composition even variable composition but not find any peak.just zig zag.
Please use the below gradient.
Time (Min) % A (0.1% TFA in water) % B (Acetonitrile)
0.01 90 10
25 30 70
35 30 70
37 90 10
45 90 10
Please optimize the wavelength with DAD / PDA (from190-230 nm)
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