Dear Rehan,

why you need to use LCMS (such a expensive technique) for solvent determination? also LCMS is not a recommended technique for simple organic solvent determination. Please use GC FID or GCMS for solvent determination as it is easy, cost effective and recommended for good specificity and selectivity for solvents.

Regards

Selvan

thanks for your reply

Yes i am using the technique GCMS of varian,But came to know from my fiend that there is some work going on on LCMS regarding this.

I agree with mr.selvan

hi chaitanya, please remove Mr.; just say selvan

Ok selvan

I would like to improve my hplc basics can u suggest any books/web sites?

HPLC Method Development for Pharmaceuticals, Volume 8

High pressure, or high performance, liquid chromatography (HPLC) is the method of choice for checking purity of new drug candidates, monitoring changes during scale up or revision of synthetic procedures, evaluating new formulations, and running control/assurance of the final drug product. HPLC Method Development for Pharmaceuticals provides an extensive overview of modern HPLC method development that addresses these unique concerns. Includes a review and update of the current state of the art and science of HPLC, including theory, modes of HPLC, column chemistry, retention mechanisms, chiral separations, modern instrumentation (including ultrahigh-pressure systems), and sample preparation. Emphasis has been placed on implementation in a pharmaceutical setting and on providing a practical perspective.

HPLC Method Development for Pharmaceuticals is intended to be particularly useful for both novice and experienced HPLC method development chemists in the pharmaceutical industry and for managers who are seeking to update their knowledge.

* Covers the requirements for HPLC in a pharmaceutical setting including strategies for software and hardware validation to allow for use in a regulated laboratory

* Provides an overview of the pharmaceutical development process (clinical phases, chemical and pharmaceutical development activities)

* Discusses how HPLC is used in each phase of pharmaceutical development and how methods are developed to support activities in each phase

Regards

Selvan

if you want read in web site: please search pdf/ppt/doc as below in the google search to get very good presentations and articles.

HPLC filetype:ppt, HPLC Method Development filetype:ppt or pdf or doc

if you need HPLC Methods use below links to see the lots of application notes, search as below in google

HPLC site:chem.agilent.com filetype:pdf

HPLC site:waters.com filetype:pdf

HPLC site:dionex.com filetype:pdf

HPLC site:shimadzu.com.filetype:pdf

i hope this helps!

Regards

Selvan

Thank u