The Lennox formulation and the Miller formulation are pretty interchangeable, I would not expect you to see any difference for most work. There is even a lower salt version at 0.5g/l which is typically called Luria Broth and also it works just fine.

Some antibiotics function better at the lower salt levels but it doesn't make a difference for Amp or Kan. But that would be one reason to go with Lennox or Luria.

Additionally if you purchase premixed media you may find the prices can be quite different for the different formulations, which is surprising given the difference is only the NaCl.

Michael J. Benedik thank you!

Dear Laura Dionisi

generally the low salt version is dedicated to some specific E.coli strain or other bacteria that are more sensitive to salt, the routinelly used is the miller formulation.

However for recombinant protein expression, i suggest you to try high cell density media that in will allow to you to reduce a lot the culture volume respect than LB and lost less time to follow the OD.

at the following link you can see a video with some information about those high cell density media

https://www.blogger.com/video.g?token=AD6v5dxhZKT6Tvug0rLIZgcg8nyl5CwzQSFiX9pLglN-foSxrhnBjoEr7ReFTWj5bbeiloMCNhNoJn2HgHEgR2eC7SYwl5TPVwlCYNniPhSpeELHsevHk1dREBGIdHqskofd9HgX-2gr

good lucjk

Manuele

Hi, DH5alpha is not affected by salt concentration, so either formulation is fine.

From my experience, the low salt LB is used for work with antibiotics zeocin, which is salt-sensitive. Of course you could have also some osmotic-sensitive strains of E. coli, but that's not the case of DH5alpha.