Dear All
I have checked OD(optical density) reading of microbe against dragon fruit peel extracted using uv vis. The wavelength use is 600nm. May I know as theorytically how this instrument get the Od reading?.Can I said, When light pass through the cuvettes,microbe will scatter the light and the changes in energy will detect by detector?
Uv-vis not have element lamp. It just consist of deuterium lamp(180-300nm) and tungsten lamp(300-800nm)