How can I take subsamples of water from a larger sample in a way that allows me to measure the iron content of the subsample?

I learned how to take subsamples with a magnetic stir bar, but I noticed iron particles stick to the bar, and I am having trouble finding a standard method to solve that problem. How can I take a...

04 February 2021 4,778 1 View

I am looking to develop a basic oral microbiome assay. Can anyone advise on culture vessel, conditions and organisms that require minimal maintenance?

I am looking to investigate oral care products with an easy-to-prepare culture of orally derrived microorganisms.

21 January 2021 3,448 1 View

PEI DNA in-vivo transfection concentration?

04 January 2021 6,908 7 View

What would be the recommended color/method for quad-IF?

I'm trying to perform quad-IF staining on frozen tissue sections on a fluorescent widefield (axioplan) microscope. I was wondering what people might suggest for colour/strategy for a co-IF...

13 October 2020 6,863 4 View

How do you remove the tolerance limits when running collinearity statistics in SPSS?

I am using SPSS syntax to determine multicollinearity with predictor variables of interest. When I enter the variables, SPSS is removing some of the variables because the minimum tolerance limit...

02 June 2020 3,639 1 View

How to distinguish different phases on an xrd pattern?

In XRD, how do you distinguish among the crystallized phase, amorphous phase, and a mixture of the two? How will the peak look like in a mixture of a crystalline and amorphous material?

05 May 2020 9,707 7 View

Are there any techniques to embed transmembrane proteins in solid lipid nanoparticles like liposomes or micelles?

25 January 2020 3,991 2 View

Specim FX17 hyperspectral camera/sensor - UAV/drone application?

Hey, I am looking into the feasibility of attaching a Specim FX17 sensor to a multi rotor system. Specs wise it looks possible, sensor is 1.4kg and Matrice M600 max payload is approx 15kg....

16 December 2019 4,169 2 View

Is the calculation of AVE and composite reliability after EFA recommended?

Greetings! The link to the Todd Grande tutorial below demonstrating the calculation of AVE and composite reliability to factor loadings appears to be popular viewing. My question is would you...

08 December 2019 711 4 View

What is the best way to model 9 continuous factors with 2 factors out of the 9 with a non-linear response, i tried using statgraphics, DOE beginner?

Good Day,this is urgent please, i want to use statgraphics trial version to study a part of my work. I am considering different stratified layers in a particular oil reservoir, i considered...

19 October 2019 3,227 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

Can RNAscope be used to quantify the mRNA expression level of certain genes?

Hello, all, I want to explore the mRNA expression level of certain genes in the brain under physiological and pathological conditions. In previous studies, the researchers usually isolated the...

18 February 2021 1,518 1 View