For example, when stimulating a cell by a growth factor, how long after adding the stimulus do we expect to see a rise in protein expression, given that none of this protein is stored inside the cell. i.e. the release is due to de novo synthesis not recycling or release?
Thierry Fischer
HI
We performed a epidermal growth factor (EGF) time course on HeLa cells and monitored gene transcription by RT-PCR. We clearly saw Fos (5 min) and Jun induction (15 min); both genes maxed at 15 min and returned to basal level 5 h after stimulation. But these genes are well denominated: immediate early genes. Translation I have no idea
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