Mohamad Musbah Almedawar

5 Questions 4 Answers 0 Followers

Questions related from Mohamad Musbah Almedawar

What are the technical differences bewtween FACS of cells and exosomes?

I'm especially looking for technical details such as using a logarithmic scale instead of linear scale. anything else? Nozzle size etc... and what are the best antibodies for endothelial exosomes.

18 March 2016 3,090 2 View

Does DAPI stain microparticles/ microvesicles/ exosomes shed by live cells?

DAPI stains A and T nucleotide rich regions, does it mean it stains only DNA not RNA? if it does stain RNA, isn't it possible that it stains microparticles/ microvesicles/ exosomes even if they...

16 August 2015 1,426 4 View

On average, how long does eukaryotic transcription and translation take?

For example, when stimulating a cell by a growth factor, how long after adding the stimulus do we expect to see a rise in protein expression, given that none of this protein is stored inside the...

24 March 2015 2,057 1 View

Does DNA methylation outside promoter region (coding sequence) affect gene expression?

I'm designing BSP primers for DNA methylation for rat BDKRB1 gene. Since gene methylation in promoter region has a regulatory effect on gene transcription, I looked up promoter regions for the...

18 December 2013 1,518 8 View

Compatible primers? Troubleshooting methylation primers for Bio-Rad PCR machine.

I was told that I need to pay close attention to the constraints laid out when using BIO-RAD machine when doing Bisulphite specific PCR and that there's a protocol or a list of specifications for...

11 December 2013 6,965 1 View