Hello,
I have been working with OE-33 (oesophageal adenocc.)cells for about a year now, I have observed that, although they usually grow quite quickly in flasks, having to split every 3 days, whenever they are in small plates (96-12 well), they have a hard time attaching and growing.
I have considered coating the plates with poly-lysin, or increasing the FCS % to 20%... any suggestions? I had them as single cells in 96 well-plates for so long, waiting for them to divide, around 1 month to see transferable colonies... And then, when i moved them into 48-WPs, the majority of them died in the process, having to wait almost just as long for those to grow.
Any experience with OE-33 and how to troubleshoot these issues?
Many thanks
Hi, Belén!
OE-33 is an epithelial-like cell line that is relatively easy to culture in flasks. However, if it grows well in flasks but encounters issues in well plates, the problem may be related to the material and surface treatment of the plates or an insufficient number of seeded cells. It is recommended to try different brands of well plates and increase the seeding density to observe any improvement.
According to the literature, clonogenic formation experiments for this cell line typically take about one month to yield observable results. Reference link: https://pubmed.ncbi.nlm.nih.gov/25701359/.
It is worth noting that 6-well plates are commonly used for clonogenic assays, as they provide a relatively uniform growth environment, facilitating cell-cell interactions and colony formation. This setup minimizes competition during growth, ensuring that each cell receives nutrients and space more evenly, thereby promoting efficient colony formation.
Hope this helps! Feel free to reach out if you need more details.
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