How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How to measure/estimate media pH inside the incubator?

I am having an issue with measuring the pH of my media. I use D-MEM/F12 media (without buffer) and try to adjust it to pH = 7.4. Since I incubate it @ 37C / 5% CO2, I added 2.5 g/L of NaHCO3...

18 January 2021 3,816 1 View

Just did Fluid Flow and Heat Transfer in a Mixing Elbow using ANSYS Fluent as per instructed but I got my net result in negative in Flux Reports. why?

Got negative results in flux reports can I get some solid answers for it, so I can reconsider on my problem, briefly can anyone describe briefly the issue that may have caused such answers? As...

08 January 2021 880 3 View

Can two seperate variables, one a strong negative correlation and the other moderate negative result into a strong positive in a multiple regression?

I selected two individual variables with very decent negative correlation coefficient to fit in the multiple regression analysis. I ran it, whilst the regression coefficients remained negative,...

03 January 2021 3,432 10 View

Is it always mandatory to report and test the validity and reliability of a newly developed questionnaire?

Hello everyone! Currently, I am doing an observational, cross-sectional study on knowledge, atittude, and practice towards thalassemia in Indonesia. My area of interest is in population studies....

16 December 2020 3,725 16 View

Lipid structures disappear upon negative stainging. Does anyone knows this effect?

13 December 2020 1,559 3 View

How can I get better picture for the attached TEM Image??

I used TEM equipped with tungsten gun to obtain attached image (several other too, like shown). Side blotting technique followed by negative staining (1% PTA for 30 sec) was used. I cannot get the...

31 October 2020 1,902 5 View

No title

29 October 2020 3,665 6 View

DNA extraction from bacterial culture?

Is it an effective method to isolate DNA from Gram-negative bacteria with only the use of a boil and freeze-thaw protocol, without any chemical purification? Also, is there a media to maintain the...

06 October 2020 6,890 8 View

How many days later the Covid-19 patient will be in negative result from his/her initial day of infection?

The opinions are different. But actually, it is essential to know how long time the Covid-19 patient will be in negative result from his/her initial day of infection.

30 September 2020 6,505 19 View