4 Questions 3 Answers 0 Followers
Questions related from Ashley Norris
I am growing BT-483 in RPMI ATCC Mod + 20% FBS + 1% PSG + 0.01mg/mL bovine insulin and they are hardly growing in culture. I perform medium changes every 2-3 days. They take over 2 weeks to get to...
10 March 2025 8,191 2 View
Hi all, I have been trying to activate isolated T cells for CAR T generation and something abnormal has happened twice. After the T cells are spiked with a 1:1 ratio of Dynabeads, a day after...
15 November 2024 6,447 1 View
Hi all, My lab has been trying to culture this cell line for the last two months and they have progressively gotten worse. We have tested them twice for Mycoplasma and have gotten negative...
27 July 2023 504 0 View
I'm trying to bank the HCC 2998 cells and have no information on them in our database. We have very few vials on hand, so I do not have a lot of wiggle-room in optimizing a medium. Does anyone...
31 May 2023 1,971 2 View