Hi,
I'm culturing immortalized mouse podocyte cell lines. Currently at differentiation stage. As seen in image below, the blue circled regions are cells that are undifferentiated, while the red arrows indicate the regions where foot processes occur, which is an indication the cells are differentiating. however, the regions circled in yellow are cells with weird morphology.
I was wondering if somebody with experience dealing with cell lines could suggest the possible reasons for such a weird morphology. the incidence of such cells at the moment is maybe 1:100
Is it due to mechanical stress? (mechanical flushing of cells during trypsinaization-accidental 'scratching' of cells perhaps with the pipette?)
or god forbids, contamination with mycoplasma?
Stress seems like what is happening. We had cells like this after a chemical treatment. In time-lapse results, the cells seemed to get away desperately from something but the thing they were trying to get away was inside them. They had long processes like yours.
I would also suggest a simple DAPI or Hoechst staining to rule out Mycoplasma.
Hello Iwan
Do you culture cells are treated?
I advice you check mycoplasma contamination. Dependent on cellular type can change color, Ph media or cell morphology. Whether all is ok. the mechanical stress could be the reason.
Regards
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