Hi all,
To conduct a cancer based discovery study by NGS what is the optimal number of samples [minimum number to be precise] which would yield convincing results as it is difficult to sponsor for large number of runs which can further lead to a decent publication.
Can you please suggest?
Thanks in advance.
Ramya
This depends heavily on what type of study you are doing, and more importantly what is the expected effect size in your sample. Is this Exome or Whole-genome sequencing to look for single-nucleotide variants or copy number variation between different types of tumors, RNA sequencing to look for differentially-expressed genes, something else?
What do you mean by "a cancer based discovery study" ?
Ben
Hi Ben,
Its basically an miRNA related study. i am interested in knowing th edifferent types of miRNA's , and also for novel detection of miRNA's in particular population with a specific cancer.
Ramya
You can capture miRNA with the Illumina Small RNA Library Prep (and RNA sequencing): http://www.illumina.com/applications/sequencing/rna/small_rna.html
Regarding how many samples you will need to discover novel miRNAs in your population of interest, this will depend on what proportion of that population expresses the novel miRNAs. Do you have any idea what this might be?
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