Number of passages for the mouse choroid plexus cell line ECPC?

Alba Del Rio Serrato @Alba-Del-Rio-Serrato

09 April 2018 1 9K Report

I´m starting to basic barrier models for the CNS, and I would like to buy the ECPC-4 cell line that I have just found available in a Japanese company (RIKEN Cell Bank). Their passage number is currently 32 and there is not much information about this cell line and its stability. I have seen that it has been reported to be stable for over a year, however I am a bit sceptic about it. Anyone has experience or has previously work with this cell line?

Amos Bairoch

A recent paper seems to have had no problem with it from passage 37 to 41:

Article Modeling immune functions of the mouse blood-cerebrospinal f...

And why woud there be reasons to think it is not stable? It is a SV40 transformed cell line and there is nothing to indicate that it is inherantly unstable.

Best

Amos

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