I will check this !

Thanks,

f

For those who are still interested in that question, we have developed QuantIF, an ImageJ macro that automatically determines the total number of cells and the number of labeled cells from two images of the same field, using DAPI- and specific-stainings, respectively. QuantIF can automatically analyze hundreds of images, taking approximately one second for each field. It is freely available as supplementary data online at MDPI.com and has been developed using ImageJ, a free image processing program. It is routinely used in our labs to quantify viral infections in vitro, but can easily be used for other applications that require quantification of labeled cells.

Reference :

Handala L, Fiore T, Rouillé Y, Helle F.

QuantIF: an ImageJ Macro to Automatically Determine the Percentage of Infected Cells after Immunofluorescence

Viruses, 2019

https://www.mdpi.com/1999-4915/11/2/165