Hi everyone,
In your experience, is it necessary for CD3/CD28 to present in during nuclefection or lentivirus transduction of activated T cell?
Also, between resting and activated human T cell, which one do you prefer for these procedures?
Thanks,
Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...
03 March 2021 2,350 2 View
11 January 2021 3,284 3 View
Somewhere in papers, it is written as Mn 2p3/2 XPS spectra can be deconvoluted into four peaks at 640.83, 641.92, 642.97 and 644.56 eV assigned to Mn(II), Mn(III), Mn(IV), and the shakeup peak,...
24 December 2020 4,323 4 View
Hi everyone, I'm gonna try to detect the IL-10, IFN-y, TGF-beta1 level in the human serum by ELISA. I bought the ELISA set from the BD contain Capture Ab, Detection Ab, Enzyme reagent, Standards....
09 December 2020 3,369 5 View
wastewater with low pH
07 December 2020 2,716 3 View
Greetings, I have a short stretch of 20 aa with strong binding affinity for a compound. I am trying to de some whole cell flourescence or visible biosensing technique based on this peptide. Can...
26 November 2020 8,322 3 View
I'm trying to theoretically design a active High-Pass Filter for ECG signal with cutoff frequency Fc = 0.003 Hz...it's too close to 0 Hz and stop band frequency must be greater than 0 Hz . So...
18 November 2020 9,089 1 View
Hi, i am sudying some quantum computer science and I am really struggling to find an explanation to the question done above. Any kind of help would be wonderfull. Greets.
09 November 2020 4,657 3 View
Explain how we choice exact column during reverse phase chromatography
01 November 2020 5,968 14 View
22 October 2020 9,354 4 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
03 March 2021 5,360 2 View
Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...
01 March 2021 8,235 2 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...
25 February 2021 3,030 1 View
How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...
24 February 2021 166 6 View
Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...
23 February 2021 5,476 4 View
Hello Everyone, I have one population, sample size 200. and needed to find the correlation between Variable A and B. Also, which T-test should I choose for hypothesis testing Please advise...
21 February 2021 7,591 4 View
Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...
21 February 2021 5,724 4 View
This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...
21 February 2021 564 3 View