Do you know a good FGFR4 antibody for PDX IHC?

Dear all, I've tried many FGFR4 antibodies I saw in the literature but some of them are not useful for IHC and some are only useful for patients slides not for PDX. Do you know a FGFR4 antibody...

14 February 2021 900 0 View

How to use the bioluminescent ATP assay kit?

I need to do a mitochondrial ATP quantification assay in insects and for this, I have a bioluminescent ATP assay kit but I don't have a protocol yet. I'd like to know if you have some kind of...

24 January 2021 3,655 3 View

Sophie Germain Prize?

26 December 2020 9,273 6 View

How do you transmit and evaluate ethics and moral values in you teaching activity?

Ethics and moral values have always being an essential part of teaching and scientific research. How do you teach and evaluate these essential subjects in your daily interaction with students?

23 December 2020 5,058 7 View

Can I use the images of Collection 1 Level 2 Landsat 5 TM / Landsat 8 OLI to perform vegetation indices right away?

I'm aware that in order to perform any vegetation index like NDVI, SAVI or OSAVI you need to do some atmospheric, radiometric and terrain corrections. I'm pretty sure that Level 2 images from...

21 December 2020 4,088 3 View

Jellyfish as food?

We are investigating the acceptance of jellyfish-based food dishes in Latin America. If you are interested in the topic, please fill out the anonymous survey at the following link. Thanks in...

06 December 2020 4,266 2 View

Can someone provide me hoagland nutrients protocol modified for alfalfa?

I am looking for a good Hoagland nutrients solution protocol modified for alfalfa (stock and working). I will use it for hydroponic cultivation of alfalfa. I am not satisfied with one I have. I'll...

25 November 2020 1,791 1 View

Which is the best software to do a finite element analysis for composite materials?

we also want this composite material to be able to be modeled for mechanical testing.

19 November 2020 2,069 4 View

Why is cronbach's alpha sometimes calculated for non-unidimensional questionaries?

I've recently come across with several published articles where questionaries composed of more than one section (where every section is meant to gather data for separate variables/dimensions of...

11 November 2020 1,805 7 View

Do you know if is possible to visualize an hetero or homodimer interaction with a ligand in autodockTools?

I am recently working with a homodimer in AutoDockTools 1.5.6 but I cannot visualize the interactions once I have docked in Linux but only save the file as .docked.pdbq and then convert this with...

26 October 2020 1,988 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

Can only the spike protein of the virus provide immunity?

Virology, microbiology, immunology, vaccine

21 December 2020 424 16 View

Can somebody recommend me a decent textbook to learn more about signaling pathways and their mechanistic principles in general?

I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...

13 December 2020 5,690 5 View

Is it possible to extract DNA or RNA from paraffinized biopsies ?

My lab have some skin biopsies that were previously conserved in paraffine , and i wanted to know if it is possible to extract DNA or RNA from them.

06 December 2020 7,964 6 View

Could the desmin mutation in myofibrillar myopathy be caused by an infection?

A patient with desminopathy (mutation Thr341Pro DES in a heterozygous state) with the progression of the disease has a decrease in taste and smell, immunosuppression, and an increase in IgA in the...

02 November 2020 5,886 6 View

Whis HLA-A2 TAAs are best for DC pulsation and CD8+ cytotoxic T cell induction?

When looking to establish Ag-specific CD8 cytotoxic T cells with autologous dendritic cells, there are numerous tumor-associated Ags to choose from. Does anybody know which Ags give the best...

27 October 2020 2,068 2 View

Resuspending cell pellet after ficoll centrifugation?

In my current project I am planning to isolate mononuclear cells from peritoneal ascites of cancer patients using ficoll centrifugation. However, following isolation of the mononuclear cell layer,...

26 October 2020 5,966 4 View