I am doing in vitro transcription, my target RNA is 36nt in length. After transcription when I am running it on Denaturing gel, I am not able to see my RNA band under UV shadowing. But when I am staining the PAGE gel with SYBR gold I am observing the RNA band at correct length. For Cutting the RNA band and it's purification, I need to cut the band under UV shadowing. I have attached here the gels with and without staining.Any suggestions will be highly appreciated.