Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

Does anybody work with CoolLED in fluorescence microscopy?

I have trouble with exciting wavelengths higher than 460 nm while e.g. ex with 405 nm works. Filtercubes are correc, as I used them with my old xenon lamp before... I work on axiovert200M and with...

25 January 2021 5,201 2 View

Myeloperoxidase Colorimetric Activity Assay Kit (Sigma-Aldrich, ELISA MAK068). When do we stop the reaction?

We'd like to use this kit for brain tissue samples (rat). When do we stop the reaction, 30, 60, and 120 minutes? We don't like to test at all three stop points, because we've too many samples.

17 January 2021 6,744 2 View

URGENT: How to eliminate mold contamination from brain tissue slices?

03 November 2020 8,357 2 View

Quantitative research - hypothesis formulation necessary?

Dear all, I am planning to undertake quantitative research with a survey for my thesis. I am wondering if the hypothesis formulation is compulsory or am I able to do my research without it? My...

29 July 2020 6,145 5 View

Does anybody know if there exist research about a possible impact from military used electromagnetic waves frequencies on insects or VLF-waves?

I am very interested in the possible impact of radiation, espacially electromagnetic waves on insects. I searched several times in different online libraries, if there exists any research about...

29 April 2020 5,918 2 View

Could the new starlink satellites be dangereous for the insects due to the electromagnetic waves which they sent to the surface?

There are now many new satellites and are becoming very more ( 30 000) in the next future. I read in the internet that this satellites have not ready investigates risks on healthy and nature due...

23 April 2020 3,660 5 View

Why do I get different protein concentrations by homogenizing?

I implemented a method for protein extraction of skin samples. I did not change the procedure but over time the protein yield decreased. I homogenized skin biopsies using a bead mill homogenizer...

16 April 2020 3,170 7 View

How can I calculate the total average (or median) if I have 10 samples averages with different sizes?

I want to do some bibliography research and I have a table with the average age of 10 samples. Each sample has different size. Also I don't have the SDfor every dataset info.

07 January 2020 1,852 3 View

Can anyone recommend a database for dynamic facial expressions that also include expressions of complex emotions?

Most of the databases of dynamic stimuli for facial expressions only include faces expressing basic emotions. Is anyone aware of a database that also includes dynamic face expression stimuli on...

05 November 2019 8,263 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View