Dear all,
does anyone know if a molecule that inhibits Notch but not gamma secretase exists?
Thank you very much in advance
Elsa
Good afternoon, everyone. I am trying to locate the manual of a lighting controller, whose code is PBCON-24T-700-4 from the company DesignInnova or Photo Biosim. Does anyone know how to get it or...
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is there a specific and direct reason do tell please..
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Hii all, I am new to LabVIEW, Can anyone please share the LabVIEW code to get a voltage vs current plot for a resistor by Keithley 2460? Thanks in advance. Reshna Elsa Philip
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Climate change is a topic that should be given so much importance. How can I go forward with this topic for studying the impact of climate change on people's well being?
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Dear all, As a part of my research project, I am interested in ADAM-17/TACE and its expression in Caco-2 cell line. According to the literature that I have red until now, I don't really know if...
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Does anyone have experience in oligodendrocyte purification by immunopanning? I would like to select for GlaC and O4 but all the protocols I found use hybridoma supernatants, which I struggle to...
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I'm searching data of prescription of carbamazepine and sulfamethoxazole in some nations in some years. Who knows a web site, or a survey that can be usefull for me? The kg/year consumption I'm...
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I'm searching for some softwares (or methods) that can help me to model the fate of pharmaceuticals (or pesticides/chemicals) from runoff in agricultural soil. I tried to use RZWQM and PRZM but...
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Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
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I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
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Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
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I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
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It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
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In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
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I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
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