We have substantial base OCR and ECAR readings in the Seahorse XF24 assay of our tissues (thin pieces of tissues containing about 200,00o cells), but we could not get any responses to uncoupler, rotenone, or oligomycin!   For the same tissue, if dissociated and plated as a monolayer, we can get drug responses.   Does anyone know what is the problem?  

CJ

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