No Cq despite amplification curve in lightCylcler96 from Roche?

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Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

Increasing Area Ratio on Quality control Standard on LCMS ?

Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...

08 July 2024 3,150 2 View

Systematic reviews and/or meta-analysis and articles from predatory journals/publishers ?

Do you know systematic reviews and/or meta-analyses that have chosen to exclude articles from (or suspected to be) predatory journals/publishers in their work ?

24 March 2024 4,941 3 View

How to store insect sf9 cells?

Is it possible to keep them in -80 freezer or should we keep them in liquid nitrogen?

17 July 2023 2,846 1 View

We're looking for a complete system for producing anodized aluminum oxide (AAO) substrates from self-supported aluminum foil?

12 June 2023 3,526 2 View

Is there any free AI tool (machine learning) for the prediction of urinary tract infection based on dipstiks and sediment?

Dear members, As you know, the identification of urinary tract infection by culture is crucial for the diagnosis and treatment. However, traditional methods of culture identification can be...

04 May 2023 7,178 3 View

What company provide humanized mice?

Hi, I need humanized mouse models for flavivirus infection but I do not have the skills to produce them. Do you know of a company that can do it. Thank you very much

20 February 2023 3,058 0 View

No melting peak in DTA for an amorphous material ?

Hello everyone, I'm currently doing some DTA analysis on two phases of the same ceramic material, one is fully crystalline and the other fully amorphous. When I work with the crystalline one I...

03 July 2022 8,061 3 View

How to avoid yeast zygot clumping?

I would like to perform some microscopy experiments on yeast zygots. Unfortunetly zygots are clumping together which is not ideal condition for my experiment. I tried to add 0.1% of Tween80 with...

01 March 2022 8,606 2 View

Quantifying the uncertainty around the prediction of a logistic regression model: an available R package to estimate profile likelihood 95%CI?

Hi, I'm a fish biologist and I'm interested in assessing the uncertainty around the L50, which is the (sex-specific) length (L) at which you expect 1 fish out of 2 (50%) to exhibit developed...

21 November 2021 173 11 View

Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

In routine core analysis why we take whole rock samples, although we are taking plugs every foot?

Thanks

03 August 2024 3,567 2 View

Do you think can be any diamond bearing rocks in Eastern part of Iran near to Birjand city?

I want to know more about diamond ore deposits in Iran.

02 August 2024 7,399 0 View

Z-average size from Dynamic Light scattering can it be lower than the original probe size?

Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

01 August 2024 1,168 4 View

How to build my own lab made four point probe set up?

Hello, I'm trying to measure the conductivity of semiconductor films but since I don't have a commercial four point probe set up I would like to build one on my own in my lab. I have generators,...

30 July 2024 906 2 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

Do you think how can be cu bearing rocks with grade 10 in next to a granite body?

I want to know more about Cu deposits.

18 July 2024 8,834 0 View

Do you know best uranium bearing rocks except of alkali granites and their related sedimentary rocks?

I want to know more about U deposits.

10 July 2024 5,042 1 View

Chromosomal spreads are shrunken after fluorescence in situ hybridization (FISH), why?

Dear colleagues, We are trying to do FISH on mice PDEC metaphase spreads. We use homemade biotin labelled probes, synthetized using a nick translation kit. For hybridization, initially we were...

08 July 2024 8,687 3 View

Which could be origin of symmetrical botryoidal mineralization?

Hi Me and my collegues found a strange rock aggregates here Lunda Sul, within Kalahari sand suite. Similar formations (ferricrite, ferricrete) are found within Lunda Sul, Angola on the surface...

23 June 2024 2,507 3 View

Jincheng Liu

0.012 is the problem. Go to the analysis setting window, change the number to 0.2, the Cq will be showed.

Julien Pompon

great thanks.

then, I just need to click left on the threshold and select the fluorophore.

Karim Majzoub

use CFX from BIoRAd with SYBR green :) better software :)

that's the one I had in Singapore and, for now, I prefere Biorad soft indeed. LC96 thinks is too intelligent and does not let you have the hand on many parameters ;-)