From the manufacturer: Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of H2O to adjust the volume to 1 mL.

Thank you so much!