In mammalian cells, the non-homologous end joining (NHEJ) pathway is essential for the repair of DNA double-strand breaks (DSBs), and it includes the DNA-dependent protein kinase catalytic subunit (DNA-PKcs). The kinase domain, as well as the FAT, PI3K, and FATC domains, are only a few of the areas of the DNA-PKcs protein that are crucial for its function.

The FBR (FAT binding region) of DNA-PKcs is a 205-amino acid region that is critical for the stability and activity of the protein. However, the binding partners of the FBR region are not well characterized. the protein Nbs1 as a potential binding partner for the FBR region of DNA-PKcs. Nbs1 is a component of the MRN complex, which plays a role in DSB repair and signaling. But they’re studies that showed that the FBR region of DNA-PKcs interacts directly with the N-terminus of Nbs1 and that this interaction is important for efficient DNA repair. Also there’s another identified a protein called RAD50 as a potential binding partner for the FBR region of DNA-PKcs. RAD50 is also a component of the MRN complex and plays a role in DNA repair. According to the study, effective NHEJ depends on direct interaction between the FBR region of DNA-PKcs and the coiled-coil domain of RAD50.

I hope this helps

Sufficiency Elijah Thank you, this helps indeed. Maybe, if its not asking to much you could link the studies about those interactions with NBS1 and RAD50 ?