NGSfastq seq analysis of Human Single Fold scfv Libraries I + J (Tomlinson I + J) ?

31 August 2023 0 5K Report

I am performing the NGS seq analysis of Tomlinson I + J phage library.

The offical manual states that "Diversified (DVT) side chains based mainly on those positions which are diverse in the primary repertoire (total of 18 residues - H50, H52, H52a, H53, H55, H56, H58, H95, H96, H97, H98, L50, L53, L91, L92, L93, L94 and L96)."

But it doesn't give exact location of library insert or the codon sequence flanking the library insert as provided by other phage libraries (see attched NEB 7PhD).

Can anybody please share the sequence highlightinh the library insert regions ?

Thanks

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