Hi all,

We are investigating gastric cancer development using biopsy or ctDNA samples.

Using WES, we could find the drug-related and cancer-driven genes or mutations.

We also ordered H. Pylori probes for UREA/B CagA VacA genes, together with WES probes, to evaluate the pylori status of the samples.

We found hard to get the infection status, although most of the samples should be tested positive.

By the way, we extract the total DNA (human+bacterium) from the FFPE samples, rather than isolate the bacterium separately.

Any one can tell me how to enrich the H pylori targets?

Thanks,

Junfeng