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Questions related from Junfeng Jiang
Hi all, I am writing to request technical suggestions for improving chromatin fixation conditions in PBMC samples for Hi-C assays. Here is my current experimental context: Experimental...
31 March 2025 9,586 0 View
Hi, all We commonly used 10% formaldehyde to fix tissue for pathological usage such as IHC or FISH. It was reported that proteinase K or sonication+SDS can reduce the cross-linking level of the...
10 October 2024 904 5 View
Hi, We would like to use DSN to digest the wild templates against the mutants. Nearly all references suggest enzyme from Evrogen, I would like to know any similar ones? Best, Junfeng
30 November 2021 7,490 3 View
Hi We are dealing with enrichment of low abundance mutation in ctDNA samples. As in many papers, DSN enzyme was commonly used to eliminate the wild type molecular in ctDNA samples. However, we...
26 October 2020 2,850 0 View
Hi all, We are investigating gastric cancer development using biopsy or ctDNA samples. Using WES, we could find the drug-related and cancer-driven genes or mutations. We also ordered H. Pylori...
08 October 2020 3,190 1 View
Hi We want to use SBE to genotype the SNP we want. Any suggestions to buy the polymerase and ddNTP (without fluorescence)? We find a bit expensive from GE, any alternatives?
24 April 2020 2,729 0 View
Hi all, I am using IDT capture probe to enrich the target region of tumor samples for sequencing. However, I found Skewed GC bias in NGS data, which resulted in poor capture uniformity and lower...
10 February 2020 3,664 2 View
Hi all, I design the AS-PCR assay to genotype the SNP, plasmids were used to guarantee the three cluters. However, it is strange that the hetorozygous plasmid went to wild-type area, while the...
26 June 2016 5,512 2 View
