Junfeng Jiang

4 Questions 3 Answers 0 Followers

Questions related from Junfeng Jiang

Any similar enzyme as DSN (Evrogen) ?

Hi, We would like to use DSN to digest the wild templates against the mutants. Nearly all references suggest enzyme from Evrogen, I would like to know any similar ones? Best, Junfeng

30 November 2021 7,474 3 View

Next generation sequencing for H. Pylori?

Hi all, We are investigating gastric cancer development using biopsy or ctDNA samples. Using WES, we could find the drug-related and cancer-driven genes or mutations. We also ordered H. Pylori...

08 October 2020 3,165 1 View

Skewed GC bias in NGS data?

Hi all, I am using IDT capture probe to enrich the target region of tumor samples for sequencing. However, I found Skewed GC bias in NGS data, which resulted in poor capture uniformity and lower...

10 February 2020 3,625 2 View

Allelic qPCR genotyping?

Hi all, I design the AS-PCR assay to genotype the SNP, plasmids were used to guarantee the three cluters. However, it is strange that the hetorozygous plasmid went to wild-type area, while the...

26 June 2016 5,495 2 View