Inhaler antiviral therapy may be more effective than systemic antiviral therapy in Covid-19 patients?

... There is an urgent need for information on virus replication, immunity and infectivity in specific sites of the body. Here we report a detailed virological analysis of nine cases of COVID-19...

27 May 2020 9,549 4 View

Do microorganisms use humans as a carrier?

Every member of the human population contains approximately 10131014 cells, 70% of which are cells of symbiotic bacteria predominantly localized in the gastrointestinal tract. Sometimes , I think...

18 April 2018 1,965 14 View

What do you think about cancer test, called CancerSEEK?

More than 2 years ago, Tomasetti and Vogelstein brought that question to prominence when they tried to sort out environmental versus inherited causes of cancer. They examined the extent to which...

21 January 2018 2,088 9 View

Is the speed of cosmic inflation equal to speed of time(Cosmic inflation is time)?

According to the theory of inflation, the Universe grew faster than light and I think speed of inflation is "speed of time" at the same time.We are convicted of this inflation(time) with gravity.

12 January 2018 2,961 15 View

Did I use the Analysis of Means correctly in this example?

29 June 2017 6,208 2 View

Nichotherapy for cancer. Is metformin suitable agent?

Metformin's anti-cancerous properties have been demonstrated in various cancer cells in vitro, such as lung, pancreatic, colon, ovarian, breast, prostate, renal cancer cells, melanoma, and even in...

16 June 2017 4,888 3 View

Do transposable elements (TEs) indicate that cancer is an adaptation mechanism?

27 March 2015 8,435 22 View

Is it possible to calculate entropy of the cells? Is entropy level of chaotic systems different from random systems?

If Cellular entropy calculate in different type of cells (stem cell, differentiated cell, cancer cell..) , it provides a valuable information for carcinogenesis.

12 December 2014 397 17 View

No title

Aneuploidy, an imbalanced complement of chromosomes, was identified as a distinct feature of cancer cells more than a century ago. Indeed, ~90% of solid tumors are aneuploidy. The random gain and...

01 January 1970 8,010 21 View

Microarray analysis on non coding RNAs

While examination of the differential expression of non coding RNAs from blood samples or cell cultrues and animal models, how many times should we repeat the microarray analysis experiments ?...

01 January 1970 2,658 10 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View