Can anyone give me the supplier name for neutralizing antibody to human CD11b?
Thanks
I digest my plasmid with KpnI-NotI. I cut the expected 4kb band from the gel, purified it and then I did ligation [1:3 and 1:5 vector:insert ratio] and transformation. I found 18 transformed...
30 June 2024 3,976 0 View
My vector size is 5.1kb and insert size is 4kb. Initially, I performed digestion of the insert from a different plasmid and the vector using KpnI-NotI. I checked them on the gel, excise and...
19 May 2024 3,185 8 View
My vector size 5.1kb and insert size is 4kb. I performed double digestion of vector and insert, ran them on the gel and then extracted them from the gel. 260/280 ratio was good. Then I did...
06 May 2024 8,310 11 View
While preparing E. coli DH5alpha competent cells, most of the methods describe using E. coli DH5a at OD 0.4-0.5. Is there any specific reason for using OD 0.4-0.5? If OD is more than 1, will it be...
17 April 2024 2,297 3 View
I am looking for antibody against human cell membrane which will circularly stain the cell membrane only. Could anyone name such antibody? I want to avoid F-actin, microtubulin bcz they stain all...
22 January 2018 5,217 11 View
Is it possible to separate human M1 and M2 macrophages by flow cytometry? Has anyone done this? Could anyone provide any related literature?
09 November 2013 4,966 14 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.
07 August 2024 9,508 1 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Chimnya
05 August 2024 6,947 1 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Hello- I have two profiles in ResearchGate. One under my married name of Kristina Secnik and one under my married name of Kristina S Boye or Kristina Boye. How do I merge my publications so that...
29 July 2024 4,426 1 View
The Louvain method – named after the University of Louvain where Blondel et al. developed the algorithm – finds communities by optimizing modularity locally for every node’s neighborhood, then...
23 July 2024 6,659 0 View