Which statistical test can I use to test whether two standardized regression coefficients differ significantly?

Hello, I have conducted a multiple regression with two predictor variables and would like to find out whether the difference between the standardized regression coefficients is significant....

22 June 2020 8,788 11 View

Class imbalanced dataset for Machine Learning - how to test it?

I have a data-set with good and malicious files. Labelled as 0 and 1. In the data-set there are mor malicious files - like 3000 vs 800 good files. Nevertheless I am getting good results with...

27 April 2020 3,752 14 View

How to design a dataset of files and their "issues" for machine learning classification?

I have a few thousand files and each file has multiple "issues" after being deconstructed. They are labelled 0 or 1 for good or malicious. I think these "issues" can be a feature to determine if...

02 April 2020 2,941 2 View

Factorial Survey Experiment Analysis?

2x2x2 Design

26 November 2019 4,808 3 View

Has anyone experiences with drying plant parts (leaves, stems, roots, ears, kernels), especially of wheat plants in domestic microwave ovens?

Dear all, we need a procedure to rapid drying plant materials from field or greenhouse experiments to rapidly denaturing enzymes, kills micro-organism and stabilizes the tissues. On one hand we...

31 October 2018 5,942 9 View

No title

At the moment we hear a lot about artificial intelligence and machine learning. It is an awesome development, unimaginable completely or partially, now. How and when humans will gain the benefits...

19 September 2018 801 25 View

No title

21 July 2018 2,931 3 View

For NGS, can I use samples prepared with differerent number of cycles of PCR?

I am preparing samples for NGS, RNA sequencing. I isolate total RNA from B. subtilis, deplete it from rRNA and then make DNA libraries. Every step is quality-checked using BioAnalyser. And in the...

08 April 2018 7,871 6 View

Detaching cells from Lab-Teks. Do you have any suggestions?

Does anybody have any experience with cells detaching from Lab-Teks and how to deal with it? In one of our experiments we are not able to use gelatin or anything else. In other experiment we are...

13 September 2017 4,777 1 View

Bax protein isolation

19 June 2017 5,329 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

No title

The tested drug is a chemotherapeutic agent the type of the cell lines is colorectal cancer cell lines the cell viability was compared to the control my thought was the cells were activated by...

02 February 2021 1,256 11 View

Why is WST-1 assay giving inconsistent result?

i want to determine the cytotoxicity activities of some drugs with WST-1 assay using 293T cell lines in 96 well plate. I usually seed 10,000 cell per well and incubate for ~48 hours. I expected...

01 February 2021 8,554 3 View

What's wrong with this fronzen section of mice melamona tissues?

I met a problem when I was doing frozen section for mice melanoma tissue. Based on the confocal results I got as attached, seems like the cell structures are destroyed (looks like a network) and...

31 January 2021 8,464 4 View

Why do my fibroblasts under hypoxia conditions proliferate slower than the same fibroblasts under normoxic conditions?

I am using healthy control lung fibroblasts in a 96 hour experiment with two drugs (nintedanib and Pirfenidone) and vehicle controls for both. I have both hypoxia (approx 1% O2) and normoxia...

12 January 2021 6,260 1 View

Can we use Spray dried solid lipid nanoparticles for MTT assay?

I have synthesized Quercetin SLNs and spray dried them using Mannitol to avoid drug leakage. However, I need to perform MTT (cytotoxicity). Can I use the powder directly disperse it into media and...

08 January 2021 6,783 3 View