Does anyone know a coenzyme Q10 extraction protocol for fibroblasts?

Hi, I am looking for a coenzyme Q10 extraction protocol for fibroblasts, in order to be measured by HPLC. Most articles are very vague explaining their procedure. Thank you very much!

05 October 2020 8,245 2 View

It's available the Matlab-code from Traffic Dynamics project ?

I am a master student in Brazil and would like to know if it would be possible to share the Matlab files corresponding to this work (Matlab-code for Traffic Flow Comparison of Roundabouts and...

08 November 2019 4,703 3 View

Were can I find architectural projects for third world countries?

I am working in a arch. project for a comercial subcenter of a city in a third word country but all I find in the internet are examples of first word countries.

28 February 2019 7,058 5 View

How do you detect insulin pathway activation?

Greetings, I was wondering how to detect the activation of the insulin pathway in a cell culture. I thought about quantifying the protein expression of phospho AKT or phospho GSK3 via Western...

15 May 2018 2,388 3 View

How do you detect insulin pathway activation?

Greetings, I was wondering how to detect the activation of the insulin pathway in a cell culture. I thought about quantifying the protein expression of phospho AKT or phospho GSK3 via Western...

04 May 2018 9,410 3 View

How can I remove nucleic acid contamination from a cell free protein extract?

06 July 2016 1,377 5 View

How can I quantify an uniaxial pressure?

I am performing uniaxial pressure to a tetragonal oxide by reducing one of their cell parameters and relaxing the other two in a computational way. I found that the material is squeezed, suffering...

13 September 2015 9,111 5 View

How can I become to discrete the Kullback Divergence?

I have the Kullback Divergence in its continuous form and I need it in its discrete form.

18 December 2014 2,746 1 View

Does anyone have experience sorting platelets?

We are performing an experiment with NK cells and platelet cytotoxicity but we have troubles with platelet separation. Any recommendations?Thank you in advance

03 November 2014 2,479 7 View

Have you published yet on this topic? Can you diect me to your papers or the most relevant research on this very interesting topic?

I'm referring to the topic of your project, microagae to drop-in fuels.

01 January 1970 9,524 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

No title

02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View