Need suggestion for obtain axenic culture of cyanobacteria

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Rabbul Ibne A. Ahad

Please collect soil sample
dilute in water
plate on agar containing BG-11 medium
Allow them to grow
colony will grow
pick up single colony
put in the BG 11 medium
if still not getting single colony then do the above mentioned steps again.
i hope after that you will get single and pure culture/ isolate

Amaya Blanco-Rivero

Thanks. Did you try using any cycloheximide or antibiotic ??

No, i have not tried in cyanobacteria but tried in E.coli

Vrajesh Patel

If you want full details please go through this article. I think it covers all whayever you need to know.

Article Isolation and Purification of Cyanobacteria

Thanks Vrajesh

Thanks Rabbul