I am looking for the sequence to a plasmid called pBeh pac18. It was created in 1988 in the Hauser lab. It is not a commonly used plasmid and I cannot find its sequence online. Can anyone direct me towards it?
Just a generic question, what is the average half-life of a typical membrane protein? If you have a review off hand that could be sent to me, that would be greatly appreciated.
03 April 2019 197 3 View
I have an Orion Versa Star Pro Multi-Meter and I need to get data off of it into Microsoft Excel. We are currently using Hyper Access as a logging software but this is not meeting our needs. We...
31 December 2018 6,094 0 View
I am looking for an Olympus Cx43 model microscope that has been used or was a demo model. I would also settle for other earlier models of Olympus microscopes. I need a 100X objective, a camera...
10 November 2018 4,072 8 View
Can food grade CO2 be used in place of medical grade CO2 for a cell culture incubator? Our lab is running out of medical grade CO2 and all we have on hand is food grade. We can't order more...
05 June 2018 243 0 View
Hi, I need a good inhibitor for CDC42. I am new to working with this protein and wonder if anyone knows what inhibits it well. Thank you! Chuck
03 April 2018 3,829 10 View
I am looking for a dominant negative Wiskott Aldrich Syndrome Protein Mutant. I would prefer if it has an mCherry tag. Our lab would pay for shipping and acknowledge your contributions to any...
02 March 2018 6,303 0 View
I am trying to delete 30nt regions of a plasmid but have been unsuccessful in my PCR using overlap extension PCR. My primers range in size between 23 and 36 bp in length. I fear that my deletion...
11 December 2017 4,766 6 View
I am trying to purify DNA from a gel but yields are seemingly too low. I have loaded ~500ng of DNA onto my gel, run it, and cut it out and purified it using a Quigen Gel Extraction kit. I am only...
11 December 2017 6,436 13 View
Hello, I am relatively inexperienced with certain molecular biology techniques. I have a gene that has been cloned into a plasmid for me. I would like to leave the N and C Terminal domains intact...
10 November 2016 1,069 2 View
Hello, I am attempting to use Quikchange Mutagenesis to introduce a single nucleotide mutation into a circular plasmid. I have run the mutagenesis protocol in a thermal cycler with the following...
05 June 2016 2,608 7 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...
02 March 2021 811 3 View
I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...
02 March 2021 8,437 2 View
Please provide Book Title and author name
02 March 2021 9,059 3 View
We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...
02 March 2021 2,300 1 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...
28 February 2021 7,310 3 View
I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...
28 February 2021 2,004 3 View
Please, if Gaussian Parameter optimization is possible in this code structure
28 February 2021 278 3 View