Hello,
I am working with mouse cells, and I need to localize my protein. My protein should bind to voltage-gated potassium channel beta subunits. Can you please help me from your experiences? Which primary and secondary antibodies do you recommend for my study? My protein is GFP (green fluorescent), and I plan to conduct my study under microscopy. I have read a lot, but I'm not sure which one to use, and all of them are expensive, so I want to choose wisely.
I found people recommend to use monoantibody
thank you
Hi Ryan ..
Choosing an antibody for the voltage-gated potassium channel beta subunit will largely depend on which specific beta subunit you're interested in. However, here are some suggestions from reliable suppliers:
Remember to choose a secondary antibody that is suitable for your microscopy setup, conjugated to a fluorophore that doesn't overlap with GFP, and that is raised against the host species of the primary antibody you choose. For example, if you use a rabbit primary antibody, you might use:
- Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 594 conjugate (A-11037) from Thermo Fisher Scientific. These are just suggestions, and you'll need to consider your specific experimental design and microscopy setup when making your choice. Always check the provided datasheets for each antibody and perform appropriate controls to confirm the specificity and sensitivity of your chosen antibodies. wish you luck!