What methods do you use to isolate Rhodobacter sphaeroides from soil and water?

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Need help is coverslipping-Histology samples?

Hello, Our lab has been experiencing sporadic issues with coverslipping. We use the Sakira Tissue Tek Prisma H&E Stainer and a Tissue Tek Glas2 Automated Glass Cover slipper. Periodically,...

12 July 2024 7,016 3 View

Why are bovine satellite cells differentiated at 30% confluency?

Hi, I have been culturing bovine digest satellite cells recently. In my very latest study, i seeded it at 2.65 million cell in T75 with dmem/f12+20% fbs and 1% antian and 10ng/mL fgf and tried...

24 July 2023 9,514 0 View

Can anyone tell me the sequence for CD3 antibodies: Clone SP7, Clone EPR20752, Clone 17A2, Clone 145-2C11?

I would like to find out the amino acid sequence for a few CD3 antibodies. Does anyone also know any platform/database that I can find amino acid sequence of antibodies?

20 July 2023 5,766 1 View

Cell lysates from normal human epidermal keratinocytes, adult (NHEK) for keratin filaments quantification?

Greetings fellow researchers. I am doing experimental work on normal human epidermal keratinocytes, adult (NHEK) monolayer cell culture and I am interested to analyse the proliferation and...

17 July 2023 6,270 0 View

What is limitation of electrorotation for objects with nanometer size?

Eletrorotation was used for the measurement of electrical property of cells like dielectric constant and there were also some works for the electrorotation of nanowire, but the length was still...

14 March 2023 1,489 0 View

G2/M peak is far from G0 peak in cell cycle analysis?

Hello everyone. I have performed cell cycle analysis by FCM using FxCycle PI/RNase staining solution from Invitrogen for MSCs. I fixed my cells with 70% ethanol for 2 hours at 4 degree C before...

02 February 2023 2,302 2 View

How to avoid the MHD activities during the plasma current ramp up phase in TOKAMAK?

We know the MHD such as the NTM ,Kink mode would affect the confinement or the transport of the plasma in TOKAMAK.They have different driven free energy but not only that.In general, the MHD will...

28 August 2022 3,023 1 View

What is the proper procedure to produce Fatty Acid Chloride by using Palmitic Acid and Thionyl Chloride?

I was entrusted with the project to find out the experimental procedure of producing Fatty Acid Chloride using Palmitic Acid and Thionyl Chloride, mostly the resources online are general so I need...

01 March 2022 1,927 2 View

Why is there a difference in BCA mitochondrial protein quantification results using DPBS and storage buffer?

I isolated mitochondria using Sigma-Alrich Mitochondria Isolation kit for Cultured cells (MITOISO2) and resuspended the mitochondria pellet using their Storage Buffer (50 mM HEPES, pH 7.5,...

11 February 2022 8,392 4 View

What's your thoughts on ArcGIS Pro v2.9 accuracy assessment tool?

Hi everyone, I would like to ask for your opinion about the accuracy assessment tool on ArcGIS Pro. I am currently using the software to run drone image classification. I have done the accuracy...

10 February 2022 2,178 0 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View