What methods do you use to isolate Rhodobacter sphaeroides from soil and water?

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Machine Learning and Big Data Analytics Tools Question?

What would be the best big data analytical tools to determine changes in behavioral health (e.g., addiction behaviors)? The data used to predict behavioral health changes are secondary data which...

30 September 2020 9,522 8 View

What is a good rice variety to research on red, brown, black and white rice towards antioxidant properties and anti-alphaglucosidase on diabetes?

We will be conducting research on colored rice--red, brown, black and white rice and utilize FRAP, superoxide assay and hydroxyl scavenging assay to determine antioxidant activity and correlate it...

20 April 2020 7,334 3 View

Why does my starting culture for maxiprep not able to grow?

I have my plasmid (pCMV-4 backbone) in a DH5alpha cells and I have spread it on a LB+AMP plates (final conc of AMP= 0.1mg/ml). These plates have colonies that grew well with the expected shape and...

16 August 2019 2,866 1 View

Recovered Carbon Black from Waste Tire Pyrolysis: How to estimate the level of odor of recovered carbon black (due to VOC)?

Dear All, Currently, we are facing quality issue of our recovered carbon black(rCB) that our customer complained our rCB got unpleasant smell. I suspected those are due to volatile matter...

05 August 2019 9,657 2 View

Can e-flows be estimated based on aquatic primary producers?

River ecosystem health or aquatic ecosystem health could be determined using Index of Biotic Integrity (IBI). E-flows describe the quantity, timing, and quality of water flows required to sustain...

27 February 2019 4,451 3 View

How to tweak the attached code to accept 3 user entry as input and produce 1 binary output?

I have trained a NN with nprtool in MATLAB. However, I am planning to deploy this code with 3 user entry as input and the code will generate 1 output in binary of 1 or 0. Humbly may I ask for...

18 October 2018 2,833 2 View

What are the ways to improve the accuracy of LAMP detection?

I am currently working on a project which uses LAMP to detect Bacillus cereus. The specificity test we did earlier has no problem with the No-Template Control, however when we move on to the...

11 October 2018 5,167 2 View

LAMP NTC showing amplification while the DNA concentration do not show amplification?

I am working on a school project and we are performing LAMP on Bacillus cereus. Currently we are working on the sensitivity of the LAMP. We have performed the experiment for 3 times and for all 3...

24 September 2018 6,032 2 View

Tailed dots in 2D gel electrophoresis after CBB staining?

I have recently run IEF (7cm, PH 3-11, NL) and 2D gel electrophoresis (12.5%). After CBB staining, the dots were not shown as single dots. Most of them are tailed (see attached). I would guess...

25 March 2018 8,477 3 View

Can Salmonella enteritidis express protein under lac promoter in a designed plasmid?

I am wondering if Salmonella enteritidis can transcribed a gene which is under lac promoter? Since most of the salmonella is lacking lac operon and I am not sure the specificity of the RNA...

15 June 2017 4,682 5 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View