Hello,
I'm transforming two plasmids (dominant negative Gai2 and Gai3) from cDNA Resource Center. The protocol that came with the plasmids suggests using Top10F' cells from Invitrogen. At the moment, our lab only has Turbo Competent cells from NEB, so I used them and the protocol from NEB to do the transformation.
I got a lot of growth for dn Gai2, but for dn Gai3 I keep getting only one colony of cells. Do you think it's because I used Turbo instead of Top10F' cells or because something is wrong with the dn Gai3 plasmids since the dn Gai2 grew just fine?
Thank you!
Hi there,
The strain shouldn't make any difference: if the plasmid backbone is the same you should get roughly the same number of transformants if using the same amount of plasmid. So I suggest you check the second plasmid prep content in terms of concentration and pattern on agarose gel.
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