Hi.
Do you know the solubility of 2,4-Dinitrophenol (DNP) in water, DMSO, ethanol? I have to prepare stock solution and add it to the cells so that final concentration of DMSO or ethanol was as low as possible.
I saw the recipe like "It is prepared by dissolving 1.0 g of 2,4-dinitrophenylhydrazine in 5.0 mL of concentrated sulfuric acid and then slowly adding this solution with stirring to a solution of 7.0 mL water in 25 mL 95% ethanol". But I guess should be simpler way with DMSO, because I met in articles the usage of DMSO solution. Unfortunately, I didn't find the recipe how to prepare it (solubility in DMSO).
Thank you in advance.