NCBI genes and their SNPs?

More Mai M. Abdelmoaty's questions See All

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View

How to find ECC from radiation pattern on cst ?

i need to find ECC for 4-MIMO antenna using radiation pattern on CST

16 July 2024 7,291 1 View

What type of contaminant is it and how to decontaminate?

Hello everyone. I am culturing hIPSC in mTeSR-1 medium. I found some small round white particles persistently attached to cell colonies, and some actively moved under the 40X objective. Also,...

08 June 2024 6,797 5 View

Winprop feko software ?

please any one work on winprop feko software to simulate 5G antenna base station and network coverage send me LTE air interface file (.wst) that needed for network analysis

02 June 2024 1,229 0 View

Wide host range of one bacteriophage?

If one bacteriophage can infect more than a species of bacteria. this means that the phage might has 2 binding proteins binding to 2 different receptors on host cell surface? or one tail protein...

24 April 2024 9,227 4 View

Chloroform effect on bacteriophage stock?

Hello Dear Drs. During the purification of bacteriophage stock by chloroform method prior to centrifugation and filtration . Chloroform is able to lyse bacterial host cells . That means the...

25 March 2024 8,002 3 View

Confusion about virus DNA PFGE image ?

This dot appeard instead a visible distinct band. the concentration of the DNA was 30 microgeam/ml Can you help me please if this is a band or not and why it appears like that ?

10 January 2024 4,187 3 View

Western Blotting: help clearing high background when probing for AMPK / ACC pathway proteins?

This is my first post on the forum; I appreciate any help I can find here. Thank you so much! Our lab is exploring the role of the AMPK / ACC pathway in fatty liver disease. We drug-treated cells...

03 January 2024 3,892 1 View

How can use ImageJ program to separate RBC, WBC and Platelet/Fibrin from thrombus HE staining?

Hello, I need help from you guys. I have ischemia stroke thrombus HE image and I should use ImageJ (color deconvolution) to separate RBC, WBC and Platelet/Fibrin components in it as percentages...

30 November 2023 5,253 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Is it legal to upload a graphical abstract previously published on your paper's first page to a website such as figshare.com under CC-BY-4.0 license?

Dear friends, does anybody know that is it legal to upload a graphical abstract previously published on your paper's first page to a website such as figshare.com under CC-BY-4.0 license? Thanks.

05 August 2024 7,098 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View