1) What all mitogens can be used to increase cell proliferation apart from classical/mostly used growth factors to enhance cell proliferation?
2) Can this be universal for all cell types?
Hello Vikas Vitthal Andhale
1) Mitogens that could be used are as follows:
Phytohemagglutinin (PHA)
wheat germ agglutinin (WGA),
concanavalin A (Con A),
pokeweed mitogen (PWM)
phorbol myristate acetate (PMA)
Levan
LPS
Dextran-sulfate (DxS)
2) No. Different mitogens stimulate different cell types and their stimulation will also differ. For instance, Phytohemagglutinin (PHA), wheat germ agglutinin (WGA), and concanavalin-A (Con A) promote human and murine T cell proliferation, respectively, whereas pokeweed mitogen (PWM) stimulates both human and murine T and B cells. LPS is a non-lectin mitogen for murine and human B cells.
Levan, LPS and dextran induce a proliferative response in human lymphocytes. These three stimulants differ, however, in their mitogenic potency and in their optimal concentration. Levan induces the highest stimulation, while LPS and dextran are stimulatory to a lesser but significant extent.
Best.
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