Native protein extraction from cryopreserved, pulverised mouse tissue?

31 August 2020 0 5K Report

Can anyone recommend an efficient protocol for the extraction of native proteins from cryopreserved, pulverised mouse brain tissue? Ideally, this should also preserve membrane proteins. Thanks in advance!

Badges
Science topic

Similar topics
Chemistry
Pharmacology

More Norbert Volkmar's questions See All

Does anyone know why this article is republished using the fake address. Is it about stealing my patent?

This article is mine. It was republished by unknown person using an address (Wilson N Jones Regional Center, Sherman Texas USA. I have a patent.

10 August 2024 7,528 5 View

Aspen Plus Simulation?

Greetings When one is to perform Simulation or modelling of Pyrolysis of Biomass Waste materials using Aspen Plus software, and one has both the macromolecules breakdown (Cellulose, Lignin and...

06 August 2024 5,023 1 View

Evaluating a developed cybersecurity framework for SMEs in Nigeria?

I am humbly seeking an expert to evaluate my developed cybersecurity framework on my Master degree final project titled " Developing a framework to Enhance Organizational Cybersecurity by...

13 May 2024 3,792 0 View

For my msc project I want to build a cybersecurity framework to enhance organizational cybersecurity?

This cybersecurity framework is going to be tailored to SMEs in my country. Any suggestions please on how this framework can be evaluated as i don’t know how to go about achieving that at this...

17 November 2023 9,738 5 View

Msc Cybersecurity and Human Factors Project Topics Recommendations?

Please I need help. can someone please recommend Project topics i can choose from for my Msc in Cybersecurity and Human Factors degree. Thank you!

03 November 2023 7,880 5 View

Dear colleagues, there are two account of me: one "Norbert W. Brattig" , the other "Norbert Brattig". Please merge these bot under "Norbert Brattig"?

I hope all my publications can be find under the name "Norbert Brattig", since only in a number of oublications I used my name including my middle name. Thus, under "Norbert W. Brattig" the...

25 May 2023 6,499 1 View

In vitro lipid / cholesterol delivery to membrane preparations?

Is there an easy way to deliver lipids in vitro to crude membrane preparations? The membranes have been prepared from cell lysates by ultracentrifugation in non-denaturing, detergent-free buffer....

15 November 2022 8,241 0 View

Sucrose gradients and mass spectrometry to analyse protein complexes?

I was wondering if anyone could relate their experience with isolating and analysing protein complexes in complex samples (whole-cell lysates) using mass spectrometry on sucrose gradient...

16 March 2022 9,995 5 View

What solvent gradients can be used for lipid group fractionation by normal-phase hplc?

We a re trying to separate cholesterol, cholesterolesters, triglycerides and squalene by normal-phase hplc. We only need a crude group fractionation. However, with our solvent system consisting...

14 February 2022 6,507 2 View

Experience with artificial neural network software?

Has anyone experience with the software NeuralDesigner? Can anyone recommend this software oder recommend another software? I have to perform classification and prediction tasks with different...

10 November 2021 5,478 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View