Native gel with extra Mn2+ couldnt not polymerize enough to give good wells. Why?

11 February 2016 1 4K Report

I was making two native gels with 5mM Mg2+ in one and Mn2+ in other. Mg2+ was fine. Mn2+ gel did not give any wells and all collapsed and remaining gel solution turned different color. Please see attached picture. What chemistry is causing this?

Rafael Félix

I guess it would depend on what is your source of manganese. Mn is quite a strong reducing agent, compared with Mg. Also, if you introduce some sort of Manganese oxide in your gel prior to polimerization, it is very likely to alter the pH (each molecule of MnO4 consumes 8 molecules of H3O+), therefore altering the polymerization or even inhibiting it. Is this for RNA studies? Maybe adding the salt only to the running buffer will be a solution...

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