In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View

What are the harmful effects of these extreme rainfall on the health of newborns? What are the reasons for that?

Extreme rainfall associated with climate change is causing harm to babies in some of the most forgotten places.

01 March 2021 5,647 4 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View

How to I carry out tobit regression on STATA with Simpson index as my dependent variable?

Hello everyone, I am working on the topic; Impact of Income Diversification on the Livelihood of Small Holder Dairy Farmers. One of my objectives is to evaluate the determinants of income...

04 January 2021 1,388 2 View

What is the risk to the children by COVID 19?

Newborn babies and infants do not appear to be at increased risk of complications from the infection. Most children and adolescents with COVID-19 have mild or no symptoms and recover within one to...

28 December 2020 9,369 4 View

Anyone interested in co-authoring a book chapter?

Dear all, I've been invited by an editor to submit a book chapter on COVID-19 (not a predatory publisher) and I'm proposing to write a piece on paediatrics. I am looking for two co-authors who...

07 December 2020 6,247 9 View

What is the mechanism of RNA fragmentation?

https://www.thermofisher.com/order/catalog/product/AM8740?SID=srch-hj-AM8740#/AM8740?SID=srch-hj-AM8740 RNA Fragmentation Reagents The average size of the resulting fragments will be 60-200...

12 November 2020 4,195 2 View

Neonatal Renal dose adjustment! Guidelines or the reference that is most authentic in clinical practice.??

In the infants to the adult age group, we have been given a concrete guideline for dose adjustment on the basis of CKD-Stages or creatinine clearance. this pathway is missing in neonates but...

07 September 2020 1,731 3 View

Why COVID-19 is almost harmless to even newborn kids?

24 March 2020 5,887 13 View

How to articulate independent variables in SPSS for doing binary logistic regression in case of infant mortality analysis?

My research topic is " Factors effecting on infant mortality among the slum dwellers". For this I have to specify some independent variables which are significant or not for the occurrence of...

23 December 2019 2,390 3 View

How to articulate independent variables in SPSS for doing binary logistic regression in case of infant mortality analysis?

My research topic is " Factors effecting on infant mortality among the slum dwellers". For this I have to specify some independent variables which are significant or not for the occurrence of...

11 December 2019 2,672 3 View