Western blot protein of interest falling at a lower weight than expected?

My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...

11 February 2021 6,009 3 View

The use of n-of-1 studies for rehabilitation / therapies (i.e. non-drug/medicine studies)?

Hi there, I am eager to hear from researchers or clinicians who have used n-of-1 studies for the above purposes. They seem to offer advantages over many other study designs, particularly for when...

26 October 2020 328 5 View

Does anyone have a copy of this article?

Bartlett H, Westcott L, Hind P, Taylor H (1998) An Evaluation of Pre-Registration Nursing Education: A Literature Review and Comparative Study of Graduate Outcomes. Oxford Centre for Health Care...

07 September 2020 552 14 View

PLS-SEM or CB-SEM after qualitative work?

Hello, My research team completed extensive interviews/triads with participants on various topics. The quantitative survey is the next step in our process. From that survey we want to prioritize...

13 August 2020 828 5 View

Are there examples of non-native insects that respond negatively, or neutrally, to anthropogenic disturbance?

I've combed the literature, but am having a hard time finding any examples. Specifically, I am looking for any examples of non-native insects that respond negatively, or neutrally, to...

30 July 2020 8,920 6 View

Why is SDS-PAGE incorporated into Western Blot over native PAGE?

I know both can be used, but why is SDS-PAGE "preferred"? Does it have to do with cross-reactivity?

29 March 2020 6,000 5 View

Do anyone have the experience of testing creatinine (kidney function) by using HPLC?

In my experiment, I want to use serum (mouse) to test the creatinine level via HPLC. 1. During the preparation, I centrifuged the whole blood, and then transfer the supernatant (which is serum)...

17 February 2020 9,679 2 View

If two drugs activate the same apoptotic pathways, can they have an antagonistic effect?

Hello! I'm testing the photochemotherapeutic potential of a drug in vivo. It turned out that treatment with the prodrug caused an effect that was similar to the effect obtained after...

10 February 2020 3,001 3 View

How do you conduct a factor analysis with Multiple Imputation Output in SPSS?

I am working on my doctoral dissertation and have hit a road block with my factor analysis. I used multiple imputation for missing data (n=198 with 5% missing data), but now I am unable to figure...

05 February 2020 6,437 3 View

Why does my analysis keep excluding one of the classes?

We are analysing data on a cluster randomised control trial. We have treated the clusters (classes, N = 8) as fixed effects, thus dummy coding to create 7 different variables. This is because we...

04 February 2020 9,301 1 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View