Is anybody working on reporter assay driven by Nanog? I have been trying to construct Nanog-driven luciferase reporter using Top-Flash reporter system but could not succeed. The background is very high and nanog over-expression could not enhance the luminescence.
Maybe you could find a suitable construct on the Addgene webpage.
e.g. http://www.addgene.org/16337/
Hi,
You might contact Mieke Geens, a professor at the Vrije Universiteit of Brussels. I recently produced lentiviral vectors for her to set up such a reporter system.
Karine Breckpot ([email protected])
Rex1 is reported to be positively regulated by Nanog.
http://www.ncbi.nlm.nih.gov/pubmed/16714766
Thomas E Darga. Thank you for the link. I have emailed him long ago (twice) but have not received any response from him.
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