• S-100. Positive in 95% of melanomas. Usually negative in signet-ring cell and rhabdoid melanomas. Usually positive in desmoplastic malignant melanomas.
• NKIC3. Slightly more specific than S-100 but stains many non-melanocytic tumours.
• HMB-45 Stains between 90% and 100% of primary melanomas, 80% or recurrent and metastatic melanomas and a lower proportion of spindle cell melanomas. Stains only minority of cases ofdesmoplastic malignant melanomas.
• Melan A Rarely stains desmoplastic malignant melanomas.
• MART-1 and MITF
• Anti-tyrosinase. Sensitivity and specificity not yet adequately assessed, but appears to be less sensitive than S-100 and more sensitive than HMB-452. Stains only minority of cases ofdesmoplastic malignant melanomas.
• PNL2: a initial report shows it is of similar sensitivity to other markers.
• KBA62 is a relatively new melanoma marker.
• Anti-BRAF V600E immunostaining-using the monoclonal mouse antibody

VE1

• Five markers (ARPC2, FN1, RGS1, SPP1, and WNT2)

were selected for their potential utility as diagnostic markers

Melanoma Marker antibodies for your research by isotype, epitope, applications and species reactivity.

a unique system for rapid identification of Melanoma Marker Antibodies. 

Melanoma (LHM 3) sc-53370 mouse IgG1 FL (h) WB, IF human

Melanoma Marker (HMB45) sc-59305 mouse IgG1 FL (h) IF, IHC(P) human

Melanoma Marker (2G12) sc-69646 mouse IgG1 FL (h) WB, IF human

Melanoma Marker (NKI/beteb) sc-52704 mouse IgG2b N/A IF, IHC(P) hu

Melanoma Marker (NKI/C3)sc-52351 mouse IgG1 N/A WB, IP, IF, IHC(P) hu

Melanoma Marker (PNL2) sc-59306 mouse IgG1 FL (h) IF, IHC(P) human

Few important serum biomarkers are TA90IC and YKL-40, serum lactate dehydrogenase (LDH), S100B and melanoma-inhibiting activity.

Hi, in human serum for Malignant melanoma you can used a protein S-100 as a tumor marker.This marker has a good results.

DC-HIL/GPNMB. 

Article DC-HIL/Glycoprotein Nmb Promotes Growth of Melanoma in Mice ...

collegenase is as far as I know a marker for melanoma in the form of metaloprotease MMP

S-100 protein is very sensitive but so specific. I don't know about serum version for HMB-45 and Melan A, but check them in Google or so.

Measure the total levels of gangliosides in serum. For melanoma tissues, you can do confocal for looking into various types of gangliosides.  Gangliosides are immunosuppressive. I have worked on human melanoma and you want to search for its application in mouse system. Refer to work done by Dr. Ravindranath and Dr. Finke.

• S-100. Positive in 95% of melanomas. Usually negative in signet-ring cell and rhabdoid melanomas. Usually positive in desmoplastic malignant melanomas.
• NKIC3. Slightly more specific than S-100 but stains many non-melanocytic tumours.
• HMB-45 Stains between 90% and 100% of primary melanomas, 80% or recurrent and metastatic melanomas and a lower proportion of spindle cell melanomas. Stains only minority of cases ofdesmoplastic malignant melanomas.
• Melan A Rarely stains desmoplastic malignant melanomas.
• MART-1 and MITF
• Anti-tyrosinase. Sensitivity and specificity not yet adequately assessed, but appears to be less sensitive than S-100 and more sensitive than HMB-452. Stains only minority of cases ofdesmoplastic malignant melanomas.
• PNL2: a initial report shows it is of similar sensitivity to other markers.
• KBA62 is a relatively new melanoma marker.
• Anti-BRAF V600E immunostaining-using the monoclonal mouse antibody

VE1

• Five markers (ARPC2, FN1, RGS1, SPP1, and WNT2)

were selected for their potential utility as diagnostic markers

Melanoma Marker antibodies for your research by isotype, epitope, applications and species reactivity.

a unique system for rapid identification of Melanoma Marker Antibodies. 

Melanoma (LHM 3) sc-53370 mouse IgG1 FL (h) WB, IF human

Melanoma Marker (HMB45) sc-59305 mouse IgG1 FL (h) IF, IHC(P) human

Melanoma Marker (2G12) sc-69646 mouse IgG1 FL (h) WB, IF human

Melanoma Marker (NKI/beteb) sc-52704 mouse IgG2b N/A IF, IHC(P) hu

Melanoma Marker (NKI/C3)sc-52351 mouse IgG1 N/A WB, IP, IF, IHC(P) hu

Melanoma Marker (PNL2) sc-59306 mouse IgG1 FL (h) IF, IHC(P) human

Thanks a lot for your replies. 

Dr. Edigio, I want to know that these markers will show the same results in Balb/C mice also. 

Debarshi, do you also want to distinguish melanoma from melanocytes, or is a melanocyte-specific marker ok (like MITF, tyrosinase, TYRP1)?   All of these are ok (in tissues, not sure about serum) for a well-differentiated melanoma - which they may be, even in white mice (your Balb/c's are white, right?).   Albino melanocytes still make all the same proteins.  If some melanomas dedifferentiate in your model then you'll need a marker that is also in melanoblasts, which might be DCT or PAX3.  MAybe S100 too.  Vince Hearing at the NIH (USA) used to have good anti-mouse antibodies to nearly all of these but he retired recently, so I don't know what happened to his antibodies.  The silver or PMEL melanosomal matrix protein is the target of the NKI/beteb and HMB45 antibodies, also gp100, but they are raised against human, so not sure how good in mice.  Also will be less positive upon dedifferentiation.

Thanks a lot for your brief reply Dr. Bennett.

Mam, I'm using White Mice (Balb/c) 5-6 weeks old, actually I'm bit confused with the specificity of the markers like if i use S100B protein as a marker in my experiment so whether this marker will be specific for mice model or i can say that; will it show the same specificity as it has shown in humans?

S-100 is not a reliable marker for Melanoma at all! It is old fashioned, not specific, and works different depending on its clone...Believe me, as a pathologist with 17 years experience))

s100 sensitive but not specific hmb45 specific but not very sensitive , melanin A another name MART1 a bit more sensitive than hmb45. none are specific for melanoma as benign nevi are alo staining same way. i am not aware of any working serum markers for melanoma.

Debarshi, you asked if these markers will also be specific in mice. 

All of the melanosomal proteins will be specific for melanoma (plus naevi and melanocytes), as opposed to tumours of other lineages, in any animal.  These include tyrosinase, TYRP1, MART1/MELANA, DCT and PMEL/PMEL17, (the antigen for HMB45 etc in humans).  MITF would also be very specific - this is a melanocytic transcription factor, and is abundant enough in cells to see it.  The S100 family are also in glial cells and their tumours, so not quite so specific, even with a good antibody.  So you can check the companies for anti-mouse antibodies against any of these.

We use the S-100B assay in serum for monitoring patients with mal.melanoma and it has proved to be a reliable marker.

Hi Roy

Please Check out the review about marker of maling melonoma in attached file... I think you can find useful things... 

Thanks a lot all the respected biologist across the world for helping me out. 

Thanks all. :)

You can tryout LDHA and S100 in serum and BRAF V600E and MITF in tumor..